Generator

Part:BBa_K2014006:Design

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)


AraC-pBAD->sfGFP_W


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1289
    Illegal SpeI site found at 1424
    Illegal SpeI site found at 1601
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1289
    Illegal SpeI site found at 1424
    Illegal SpeI site found at 1601
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1289
    Illegal SpeI site found at 1424
    Illegal SpeI site found at 1601
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1289
    Illegal SpeI site found at 1424
    Illegal SpeI site found at 1601
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

We were looking for the best method to optimize gene expression in E.coli.


Source

The AraC-pBAD promoter is a fragment of E.coli K-12 genome. We used one of the sfGFP permutation, with optimized codon sequence.

References